RESEARCH

The journey from  EGG to FROG!

Meis3: A TALE-Family Homeobox Protein Controls Posterior Neural Cell Fates in Vertebrates

A. Over-expression of Meis3 protein INHIBITS FOREBRAIN,  while ENHANCING HINDBRAIN cell fates.

B. Meis3 protein knock down INHIBITS HINDBRAIN, PRIMARY NEURON & NEURAL CREST fates, while EXPANDING the FOREBRAIN.

C.  Meis3 Protein acts downstream to mesodermal Wnt3a protein to caudalize the nervous system.

 A

 Control

Ectopic Meis3 Expression

B

 Control

Meis3 Protein 

Knock Down

C

 

Meis3 protein restores posterior neural cell fates in the absence of canonical Wnt signaling.

 
Left Panel:
Morphology of mid-late neurula embryos (a) injected at the one-cell stage with either Wnt3a Morpholino (MO) (b), or Meis3 mRNA (c), or doubly-injected with both (d). Neural plate folding and convergence extension-elongation is strongly-inhibited in Wnt3a morphant embryos (b); Meis3 co-expression rescued this phenotype (d). All embryos are viewed dorsally, and oriented with anterior at the top, posterior at the bottom.

Right Panel: In situ hybridization of mid-late neurula embryos (a,e,i,m,q) injected at the one-cell stage with either Wnt3a-MO (b,f,j,n,r), or separately with Meis3 mRNA (d,h,l,p,t) or doubly-injected with both (c,g,k,o,s). Anterior-forebrain-specific XAnf1 expression is posteriorly expanded in Wnt3a-morphants (b), and suppressed by Meis3 co-expression (c,d). Expression of the posterior neural markers Krox20 (hindbrain), HoxB3 (hindbrain), N-Tub (primary neuron) and FoxD3 (neural crest) is inhibited in Wnt3a-morphants (f,j,n,r), but rescued by Meis3 co-expression (g,k,o,s). All embryos are viewed dorsally, and oriented with anterior at the top, posterior at the bottom.

 

Additional Research Projects in my Lab!

A. Regulation of Wnt-Signaling During Early Development

B.  Meis3 Protein and the Neural Crest

C.  Role of FoxD Proteins in the Mesoderm and Neuroectoderm

D. The Role of Pou91/Oct91 "Oct3/4" Proteins in Early Xenopus Development.

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